First, let me state that I have not driven this route. I did check on Good Sam's Trip Planner to see what it recommends. It shows taking I-75 through Cincy to I-74 NW to I-275 NE to Rt. 27. Again, I don't know how good this route would be. You would think that Trip Planner knowing that we are in an rv (mine is set up for 10' 8" high travel trailer) would plan a good route. I did have one experience where Trip Planner took me through the Tail of the Dragon in TN on my way to NC. That was one to write home about ;)
Hope you have a good trip.