Route Advice. 75 or 24 and 65

I now understand your situation. A little more info in your OP would have been helpful. Since this is a one time trip,and based on your circumstances, I would select the I-75 option, as it is a little flatter and traffic through Knoxville will be easier than Nashville. Keep your truck under 50 mph on the hills and below 65 on the flat, and you should have no issues. Most semi's slow to these speeds when climbing and descending steep hills, so you will still be in the flow. Stay in the right lane and make people pass you. Make sure your truck's transmission and brakes (including the trailer) are in good working order before departing. Make sure your truck has a transmission cooler and fresh oil(15K miles or less). An engine oil change may also be in order. Keep an eye on your engine temp (very important) and don't be afraid to pull over and let it cool down when necessary. You didn't say what kind of trailer your towing. If it's a TT, make sure you have the correct hitch and weight distribution system, because your tow rating is dependent on a "properly equipped tow vehicle". Lastly, don't get in a hurry and you should be OK.