Many variables but wind is the biggest with speed and gas brands 2nd and 3rd.
Unscientific but this has been my general experience.
Wind- biggest with my MPG as low as 6.5 and as high as 9 depending on direction and wind speed.
Speed - I tried 60 mph for a few trips and the average was a .4 mpg gain over rolling at 70 or whatever traffic was doing. I did not like being a rolling road block and am much more comfortable at 65 to 70 depending on traffic.
Gas-Changing gas brands caused on average a difference of .5-.8 mpg. I get best with Shell or Exxon. Worst with Sam's Club and Murphy plus a slight knock. BP, Speedway, and Citgo OK.
Your experience may vary. Randu