I would not suspect the trans for a bump, it should be the opposite of a bump if trans is going out, but I digress...
Trans fluid can be "changed" but you only change some of it, so the new mixes with the old and is immediately dirty, so unless you see burnt fluid you can't go by the cleanliness of it.
Do you monitor trans temps?
One misfire should not bump, should be barely discernible but I would surely start with the engine plugs.
Mostly, you need to ask an F150 forum, we don't know much here, but do yell at kids to get off our lawn a lot. The Ford forums are slammed with lemons.