Like stated, prolly won't hurt to pop a couple small holes in it. You'd be drilling in the top flange which is typically in compression except when the trailer bucks over a bump, but that stress is going to be less than the constant tension stress on the bottom flange from the trailer loading. Plus the flanges are doing more for lateral stability and direct tension on the frame than holding up the weight of the trailer. The web provides most of the vertical strength.
Top flange, best place to reduce the section (drilling a hole), neutral axis of the web next, bottom flange next, top of web next, bottom of web worst spot. In general of course, without having calced the stresses and designed the beam.