I'm confused by your first statement........
So I'm winterizing my trailer today and at first I don't know where the darn water heater bypass valveis. I finally locate it under an access panel which is under the mattress to the front queen bed. I proceed to turn the valve off so NO cold water should be hitting the water heater.
Turning the valve OFF would be closing it. So what valve did you close?
Do you have a 1 valve system on WH

Valve parallel..cold water goes into WH. Valve turned 90 degrees...cold water bypasses WH and 'check valve in HOT outet stops flow into WH
Do you have a 2 valve system on WH

Both valves have to be turned 90 degrees to bypass WH.
Sounds like the anti-freeze flowed backwards into WH thru HOT outlet.
Either bad check valve OR you have another valve that needs to be operated