Coronavirus
I thought I’d share some thoughts about the coronavirus threat that is occupying the news now. I’m not convinced we’ll get accurate information as some deny there is a threat while others may tend to...
Forum Discussion
Here is a preprint on a serological test for SARS-CoV-2 across multiple animal species. This test can be used to determine if a pet (or any other animal) has had Covid-19.
Here's how it works. The receptor binding region of the virus (portion of the spike that binds to cells to start the infection) is coated in a plate (test device). Serum from the possibly affected animal is added to the plate and any antibodies against the SARS-CoV-2 spike protein binds to the material coated on the plate. The excess serum is washed out and a species specific detection antibody is added to the plate. Any antibodies that bind to the SARs-CoV-2 material in the plate AND is the same species as the detection antibody will produce a titer signal. The same titer plate can be used for any species; only the detection reagent needs to be selected for the species being tested.
Multi-species ELISA for the detection of antibodies against SARS-CoV-2 in animals
I forgot to note that this is of interest to the medical community to enable determining what animal species might become reservoirs for this virus.
Here's how it works. The receptor binding region of the virus (portion of the spike that binds to cells to start the infection) is coated in a plate (test device). Serum from the possibly affected animal is added to the plate and any antibodies against the SARS-CoV-2 spike protein binds to the material coated on the plate. The excess serum is washed out and a species specific detection antibody is added to the plate. Any antibodies that bind to the SARs-CoV-2 material in the plate AND is the same species as the detection antibody will produce a titer signal. The same titer plate can be used for any species; only the detection reagent needs to be selected for the species being tested.
Multi-species ELISA for the detection of antibodies against SARS-CoV-2 in animals
I forgot to note that this is of interest to the medical community to enable determining what animal species might become reservoirs for this virus.
