We towed our 5th wheel several times through Louisvillelast year during construction. Just pick a lane and take your time. The last time (before the bridge opened) there was some stop and go traffic but we were not delayed long. How far south are you going on I-65? If continuing on through Nashville, I would take the Briley Parkway exit and continue on that until you reach I-24 and once on I-24, I-65 is just a mile or so down the road. To me the Nashville construction is many times worse than the Louisville construction. Briley Parkway will allow you to miss all that. Just a little more info if you are continuing south.