Towing 16,000# Gas 6.9mpg, diesel 10mpg 116,000 miles needed towing to pay off the diesel upgrade Empty Gas 16mpg, diesel 19.9mpg 650,000 miles needed to pay off the diesel Video
There isn't any 2500 that can pull 16,000 pounds without being grossly overloaded, so what could happen if there was a hypothetical way to load the truck in that manner does not matter.
A 16,000 lb 5th wheel is going to have enough pin weight to bust the GVWR on the 2500 truck before you even get inside.