I don't know about the 2000 model year, but the owners manual for my 08 Frontier (King Cab, 4WD, 6sp manual) states that automatics and all 4WD models cannot be towed with the wheels on the ground. It does state that 2WD models with a manual xmission can be towed with a limit of 60 mph and 500 miles max. Nothing about starting the engine after the 500 miles so if I were to tow it, I would definitely start/drive it before the 500 mile mark.
If you do not have an owners manual I'd find one and check it out further. Good luck and BTW, it is a great little truck.
Lou
05 Travel Supreme Envoy
